Nucleolar / Nucleoli 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in ICC, IF, IHC 和 ISt. Suitable for 人.
Quick Overview for Nucleolar / Nucleoli 抗体 (ABIN6153716)
抗原
Nucleolar / Nucleoli
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunostaining (ISt)
克隆位点
NM95
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原理
- Mouse Monoclonal anti-Nucleolar / Nucleoli (NM95), Purified, with BSA
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产品特性
- This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
- Purified
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免疫原
- Nuclei of myeloid leukemia biopsy cells
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亚型
- IgG1 kappa
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anti-Nucleolar / Nucleoli antibody
适用: 人 ICC, IF, IHC, ISt 宿主: 小鼠 Monoclonal NM95 unconjugated
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应用备注
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Does not react with mouse, rat or cow, others not known
- Immunohistology formalin-paraffin 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunocytochemistry acetone-fixed cells 0.5-1.0 μg/mL
- Immunofluorescence 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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Any human cells. Tonsil
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 200 μg/mL
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缓冲液
- PBS/0.05 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Nucleolar / Nucleoli
抗原
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